Biodiesel production as an alternative for energetic valorization of biomass from poultry wastewater through <i>Chlorella vulgaris</i> and Novozyme 435

Abstract This experiment used valorized biomass of Chlorella vulgaris obtained by a culture in poultry wastewater to successfully produce biodiesel through enzymatic transesterification with 1% Novozyme 435 ( Candida antarctica lipase B, CAL B). To increase % biodiesel conversion utilizing valorized biomass lipids, the study sought to determine the ideal temperature (35°C and 45°C) and alcohol:oil molar ratio (6:1 and 9:1) for the transesterification reaction (two‐factor experimental design with two quantitative levels). It was found that the temperature of 35°C and the molar ratio of 9:1 are the temperature and molar ratio conditions that improve the conversion to biodiesel, achieving a % biodiesel conversion of 82% in 8 h of reaction ( p &lt; 0.05). Fatty acid methyl esters (FAME) were characterized by gas chromatography demonstrating the production of biodiesel, the parameters acid number, density, cloud point, refractive index, and flash point complied with ASTM standards, the rheological behaviour of the biodiesel obtained was Newtonian ( n = 1).