CUsKit: a test to revolutionise the evaluation of the disinfection process

The overuse of surface disinfectants has been shown to lead to increased microbial resistance, often due to their incorrect application. The aim of this work was to develop and investigate the use of an indicative detection kit for hydrogen peroxide-based disinfectants on porous and non-porous surfaces. The detection kit allows for the detection of the disinfectant up to 7 days after its application, resulting in a colour change from transparent to yellow-orange on both on porous and non-porous surfaces. Concurrent antimicrobial tests were performed to establish the correlation between the colour change and the retention of the disinfectant on the surfaces, thereby confirming the maintenance of its antimicrobial efficacy. KEY POINTS: Increased microbial resistance often results from overuse of disinfectants. CUsKit is a hydrogen peroxide-based disinfectant detection kit. CUsKit is an excellent option to prove the presence of disinfectants.

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Publication Details

Journal
Applied Microbiology and Biotechnology
Published
2025-03-22
DOI
https://doi.org/10.1007/s00253-025-13456-8
Primary Topic
Dental Research and COVID-19
Type
article
Field-Weighted Citation Impact
0.00

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article

CUsKit: a test to revolutionise the evaluation of the disinfection process

Isabel Ferreri, Mariana Henriques, Isabel Carvalho, Débora Castro
Applied Microbiology and Biotechnology
Dental Research and COVID-19
article

CUsKit: a test to revolutionise the evaluation of the disinfection process

Isabel Ferreri, Mariana Henriques, Isabel Carvalho, Débora Castro
article en

Abstract

The overuse of surface disinfectants has been shown to lead to increased microbial resistance, often due to their incorrect application. The aim of this work was to develop and investigate the use of an indicative detection kit for hydrogen peroxide-based disinfectants on porous and non-porous surfaces. The detection kit allows for the detection of the disinfectant up to 7 days after its application, resulting in a colour change from transparent to yellow-orange on both on porous and non-porous surfaces. Concurrent antimicrobial tests were performed to establish the correlation between the colour change and the retention of the disinfectant on the surfaces, thereby confirming the maintenance of its antimicrobial efficacy. KEY POINTS: Increased microbial resistance often results from overuse of disinfectants. CUsKit is a hydrogen peroxide-based disinfectant detection kit. CUsKit is an excellent option to prove the presence of disinfectants.

Applied Microbiology and BiotechnologyVol. 109(1)
Centro Tecnológico das Indústrias Têxtil e do Vestuário de Portugal (PT), Instituto Piaget (PT), University of Minho (PT)
Universidade do Minho, Fundação para a Ciência e a Tecnologia
Openalex Percentile: Top 90%
Dental Research and COVID-19
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CUsKit: a test to revolutionise the evaluation of the disinfection process — Isabel Ferreri, Mariana Henriques, et al. · Applied Microbiology and Biotechnology (2025) | TGRS Research Map | TGRS