Effects of dietary metabolizable energy density and inclusion of oxidized soybean oil on the growth performance, serum biochemical parameters, redox status, and wooden breast incidence of broilers

Dietary nutrients not only affect the growth and pectoralis muscle production of broilers but also are related to the occurrence of pectoralis myopathies, such as wooden breast (WB) and white striping. We investigated whether dietary metabolizable energy (ME) densities and inclusion of oxidized soybean oil could affect growth performance, serum biochemical parameters, redox status, and incidence of WB in broilers. One hundred and forty-four 21-d-old Arbor Acres male broilers were assigned into four experimental diets using a 2 × 2 factorial arrangement, including two ME densities (3,100 and 3,220 kcal/kg) and two soybean oil types (Fresh oil with peroxide value = 10.23 mEq/kg or oxidized oil with peroxide value = 122.93 mEq/kg). The experiment lasted for 21 d. The results demonstrated that high ME diets increased broiler body weight gain (BWG) by11.02%, feed intake (FI) by 4.98%, and abdominal fat percentage by 17.92%, while decreased feed-to-gain (F/G) ratio by 3.39% (P < 0.05). Oxidized soybean oil diets increased F/G ratio by 2.92% (P < 0.05) and had a tendency to decrease BWG of birds (P = 0.08). Simultaneously, high ME diets increased concentrations of serum triglyceride, malondialdehyde, and protein carbonyl (PC) (P < 0.05). Concurrently, oxidized soybean oil elevated activity of serum aspartate aminotransferase and concentrations of glucose, high-density lipoprotein, and total bile acid (P < 0.05). Meanwhile, oxidized soybean oil diets increased PC concentration and total antioxidant capacity activity in serum of birds (P < 0.05). Moreover, the high ME diet containing oxidized soybean oil decreased serum albumin and low-density lipoprotein levels (P < 0.05). Additionally, the incidence of WB was increaseed in broilers fed with high ME diets compared to the lower ME diets (57.97% vs. 33.80%; P < 0.05), and the incidence of WB in the oxidized soybean oil group was higher than that in the fresh soybean oil group (50.73% vs. 40.85%; P < 0.05). Meanwhile, the incidence of moderate and severe WB myopathy was highest in broilers fed high ME diet with oxidized soybean oil, which led to a substantial increase in cellular vacuolization, widespread inflammatory infiltration, and a marked thickening of the intercellular matrix, indicating more severe degradation of myofiber tissue. Thus, we concluded that the combination of high ME and oxidized soybean oil in diets negatively affected broiler growth performance, serum biochemistry, and redox homeostasis, and significantly elevated overall WB incidence and exacerbated pathological severity.