Characterization and comparison of <i>Schizosaccharomyces pombe</i> <i>cdc15</i> temperature-sensitive mutants.

The F-BAR protein Cdc15 is essential for cytokinesis in the fission yeast <i>Schizosaccharomyces pombe</i> , playing a key scaffolding role and connecting the actomyosin-based cytokinetic ring to the plasma membrane. Here, we compared <i>cdc15</i> temperature-sensitive mutants isolated in multiple genetic screens. We determined the mutations within each <i>cdc15</i> mutant allele and analyzed their growth at different temperatures. Additionally, we report a new <i>cdc15</i> allele that highlights the requirement for Cdc15 in the recruitment of the early secretory pathway to the cellular division site. The new mutants described here expand the toolkit for studying cytokinesis in <i>S. pombe</i> .