Design and Proof of Concept of Novel Dual and Triple Vectors for Discovery of Antibody Molecules Fused to Fc

Phage display is a widely used technique for the discovery of antibodies because of the high throughput identification of target binding proteins from large genetic libraries. However, reformatting the phage expressed binding fragment to a full-length antibody often impacts binding properties, necessitating post-selection engineering and screening in a cumbersome process with multiple cloning steps. Using a specialized dual vector system named pFJ011, antibody fragments can be expressed on the phage surface fused to human (hu) Fc genes and can also be expressed as a bivalent antibody Fc fusion in E. coli.