Highly flexible, precise dielectrophoretic, AI image analysis enabled sorting of cells in a microfluidic system
Continuous flow cell sorting based on image analysis is a powerful concept that exploits spatially resolved features in cells for sorting them. However, most of the systems published so far are very complex, offer only limited image quality and/or are limited to a specific imaging modality. We present a low-complex microfluidic microscope add-on that allows any microscope to be turned into an image-activated cell sorter capable of sorting cells by previously inaccessible features in cells. The cells are handled directly, using negative dielectrophoresis (DEP) at a sample volume throughput of ca. 1 μl/min, utilizing low flow velocities around 1 mm/s, enabling fluorescence microscopy-based image acquisition and AI-based sorting, and reducing shear stress on the cells.
Authors
- Erik Beckert (ORCID: https://orcid.org/0000-0001-8418-6655)
- Neus Godino (ORCID: https://orcid.org/0000-0002-5836-2608)
- Michaela Benz (ORCID: https://orcid.org/0000-0003-0625-4418)
- Julia Hetzel
- Felix Pfisterer (ORCID: https://orcid.org/0000-0001-7508-0701)
- Michael Kirschbaum (ORCID: https://orcid.org/0000-0003-3686-8349)
- Volker Bruns (ORCID: https://orcid.org/0000-0001-7693-3542)
- Falk Kemper (ORCID: https://orcid.org/0000-0001-6717-3350)
- Norbert Danz (ORCID: https://orcid.org/0000-0002-8927-9284)
- Rosalie Kletzander
- Tobias Gerlach (ORCID: https://orcid.org/0000-0002-9153-2680)
- Thomas Schoenfelder
- Sarah Schulenberg
- Michael Schmück-Henneresse
Institutions
- Fraunhofer Institute for Applied Optics and Precision Engineering (DE)
- Fraunhofer Institute for Integrated Circuits (DE)
- Fraunhofer Institute for Cell Therapy and Immunology (DE)
- Charité - Universitätsmedizin Berlin (DE)
Publication Details
- Published
- 2025-03-20
- DOI
- https://doi.org/10.1117/12.3043648
- Primary Topic
- Microfluidic and Bio-sensing Technologies
- Type
- article
- Field-Weighted Citation Impact
- 0.00