Development of biochemical standards for high wavenumber Raman microspectroscopy and fiber-optic Raman applications (Conference Presentation)

Raman spectroscopy (RS) is a non-invasive and label-free technique capable of quantifying the biochemical composition of cells and tissues. With the increasing use of high wavenumber (HW) RS for translational applications, there is a need to standardize and ensure the repeatability of acquired and processed Raman data. In this study, we developed a method for fabricating tissue-mimicking HW Raman standards with well-defined biochemical composition and spectral profiles. This method and the resultant biochemical standards were verified to provide quantifiable, repeatable, and accurate spectral profiles as measured by a Raman microscope and a clinical fiber-optic RS system. Ultimately, this method can ensure accuracy and consistency of Raman spectral data from different RS systems and preprocessing methods, representing another important step in the clinical translation of Raman spectroscopy.