Analysis and larvicidal activity of tinospora rumphii boerl (Makabuhay) stem against mosquito larvae

This study investigates the larvicidal potential of Tinospora rumphii Boerl (Makabuhay) stem extract against Aedes aegypti larvae, assessing its viability as an eco-friendly insecticide. Phytochemical analysis confirmed the presence of bioactive compounds, including alkaloids, steroids, and saponins, known for their insecticidal properties. The stem extract was tested at concentrations of 1%, 5%, 10%, and 20%, and mortality rates were recorded at 24, 48, and 72 hours. ANOVA revealed significant differences in mortality rates across concentrations, with the 20% extract causing 98% mortality. Pearson’s correlation analysis demonstrated a strong positive relationship between phytochemical concentration and larvicidal activity. Eco-toxicity tests showed minimal adverse effects on non-target organisms, and the extract exhibited biodegradability. These results support T. rumphii as a promising natural alternative to chemical insecticides for mosquito control. Future studies should focus on isolating active compounds and optimizing application methods for broader use in integrated pest management strategies.