Optimizing Raman modalities in PDMS-based microfluidic devices for cancer detection and prognostic applications (Conference Presentation)

3D culture on microfluidic devices holds great potential for in vivo behavior representation, but faces inherent challenges, particularly in distinguishing cancerous from healthy tissue. We propose here to use Raman spectroscopy on microfluidic chips to achieve on-chip tissue differentiation. This study optimizes Raman spectrum acquisition for better signal-to-noise ratio and acquisition speed, examining PDMS parasitic spectrum, thickness, probe distance, sample size, signal specificity, and intensity. Results show an optimal Raman shift window for PDMS acquisitions, with ideal PDMS thickness under 4mm, sample distance of at least 0.50mm, and sample thickness no more than 1mm. These configurations enhance signal detection accuracy.