Induction of microspore embryogenesis in bread wheat by mannitol pre-treatment is associated with the disruption of endogenous hormone balance and substantial accumulation of auxins
The lack of ABA-driven stress defence responses may be one of the reasons for the low effectiveness of ME induction in winter wheat microspore cultures. Low cytokinin level and a disturbed auxin/cytokinin balance may then be responsible for the morphological abnormalities observed during the next phases of embryogenic microspore development. One possible solution is to modify the hormonal composition of the induction medium with thidiazuron identified as the most promising component.
Authors
- Monika Krzewska (ORCID: https://orcid.org/0000-0003-3563-4727)
- Iwona Żur (ORCID: https://orcid.org/0000-0002-2038-8554)
- Przemysław Kopeć (ORCID: https://orcid.org/0000-0002-0008-4860)
- Dorota Weigt (ORCID: https://orcid.org/0000-0002-1041-4341)
- Lenka Plačková (ORCID: https://orcid.org/0000-0003-2537-4933)
- Ewa Dubas (ORCID: https://orcid.org/0000-0002-4908-4052)
- Karel Doležal (ORCID: https://orcid.org/0000-0003-4938-0350)
- Agnieszka Springer (ORCID: https://orcid.org/0000-0002-9181-2762)
Institutions
- Franciszek Górski Institute of Plant Physiology (PL)
- Czech Academy of Sciences, Institute of Experimental Botany (CZ)
- University of Life Sciences in Poznań (PL)
- Palacký University Olomouc (CZ)
- Polish Academy of Sciences (PL)
Publication Details
- Journal
- BMC Plant Biology
- Published
- 2025-03-22
- DOI
- https://doi.org/10.1186/s12870-025-06389-x
- Citations
- 4
- Primary Topic
- Plant tissue culture and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 3.22