Spontaneously blinking spiroamide rhodamines for live SMLM imaging of the plasma membrane
We have developed spontaneously blinking fluorescent probes based on the reversible spirolactamization of rhodamine, to efficiently image the plasma membrane (PM) of live cells with enhanced resolution using SMLM. This study demonstrates that the blinking efficiency of spiroamide PM probes is not solely governed by their p<i>K</i><sub>a</sub>; the presence of a charged polar group on the amide should also be taken into account.
Authors
- Aurélie Perrier (ORCID: https://orcid.org/0000-0002-0516-9986)
- Sophie Walter (ORCID: https://orcid.org/0000-0001-6003-8914)
- Sonia Pfister (ORCID: https://orcid.org/0000-0002-1134-3231)
- Mayeul Collot (ORCID: https://orcid.org/0000-0002-8673-1730)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université Paris Cité (FR)
- Sorbonne Paris Cité (FR)
- Laboratoire Interdisciplinaire des Énergies de Demain (FR)
- Laboratoire d'innovation moléculaire et applications (FR)
- Université de Strasbourg (FR)
Publication Details
- Journal
- Chemical Communications
- Published
- 2025-01-01
- DOI
- https://doi.org/10.1039/d5cc00151j
- Citations
- 3
- Primary Topic
- Molecular Sensors and Ion Detection
- Type
- article
- Field-Weighted Citation Impact
- 2.27