Diamond-modified Concentric Interdigitated Electrode DNA Biosensor forIdentifying Mycoplasma pneumoniae

Introduction: Mycoplasma pneumoniae is a bacterial infection and the primary causative agent for pneumonia, which poses a significant health burden and strains the medical industry. Elderly individuals, people with compromised immune systems, those with lung diseases, sickle cell disease, and children are particularly vulnerable. Method: To replace traditional methods, it is essential to develop an accurate method for detecting pneumonia for early diagnosis and improved treatment outcomes. In this research, a highly sensitive nanomaterial-modified DNA biosensor was developed on a concentric interdigitated electrode (concentric-IDE) sensor for diagnosing pneumonia. The sensor surface was modified with diamond nanoparticles, followed by the attachment of captured DNA to the IDE through an amine linker. On these surfaces, specific target DNA was detected at concentrations as low as 1 pM [y = 4.2x - 1.58, R² = 0.9908]. Results: Furthermore, selective DNA was identified in mixed samples containing single- and triple-mismatched DNA sequences, with current responses increasing as target DNA concentrations increased. Control experiments performed with unrelated capture and target DNA did not result in increased current, indicating the specific detection of the target DNA. Conclusion: This biosensor could be widely applied in point-of-care settings for diagnosing pneumonia, particularly in rural areas.