Identified five variants in CFTR gene that alter RNA splicing by minigene assay
Our study employed a minigene system, which offers great flexibility for assessing aberrant splicing patterns when patient mRNA samples are not accessible, to investigate the effects of exonic variants on pre-mRNA splicing. Our experimental outcomes highlight the importance of analyzing exonic variations at the mRNA level.
Authors
- Zhi Wang (ORCID: https://orcid.org/0000-0002-5002-5025)
- Fengjiao Pan (ORCID: https://orcid.org/0000-0002-4477-9760)
- Bingying Zhang (ORCID: https://orcid.org/0000-0003-4774-9409)
- Leping Shao (ORCID: https://orcid.org/0000-0003-3493-2714)
- Xuyan Liu (ORCID: https://orcid.org/0000-0003-4341-6930)
- Ran Zhang (ORCID: https://orcid.org/0000-0002-9085-1639)
- Yan Zhang (ORCID: https://orcid.org/0000-0003-2189-0244)
- Ning Xu (ORCID: https://orcid.org/0000-0002-3873-0506)
- Yiyin Zhang (ORCID: https://orcid.org/0009-0007-4726-1661)
Institutions
- Qingdao University (CN)
- Weifang Medical University (CN)
- Peking University (CN)
- Affiliated Hospital of Qingdao University (CN)
- Qingdao Municipal Hospital (CN)
- Qingdao Eighth People's Hospital (CN)
Publication Details
- Journal
- Frontiers in Genetics
- Published
- 2025-03-20
- DOI
- https://doi.org/10.3389/fgene.2025.1543623
- Citations
- 1
- Primary Topic
- Cystic Fibrosis Research Advances
- Type
- article
- Field-Weighted Citation Impact
- 1.52
Funders
- Innovative Research Group Project of the National Natural Science Foundation of China