AI-based Structural Study of CDKN2A Inhibiting MDM2-p53 via AlphaFold2

The potential role of CDKN2A in substituting MDM2 in its binding with p53 is investigated in this study, emphasizing the significance of wild-type p53 in cancer suppression and its potential contribution to reducing cancer incidence. The protein sequences of CDKN2A, MDM2, and p53 were obtained from the UniProt database and input into AlphaFold2 to predict their three-dimensional structures. Subsequently, potential binding sites within these structures were analyzed using PLIP software. The results provide new insights into the role of CDKN2A in regulating the stability of p53, suggesting that CDKN2A may substitute for MDM2 in its interaction with p53. This research advances the field of structural biology and offers new tools and perspectives for drug discovery and biomedical research.