Regulation of WNT16 in bone may involve upstream enhancers within CPED1
WNT16 stands up as an essential gene for bone homeostasis. Here, we present new evidence of the functional role of a particular region within WNT16. Performing 4 C chromatin conformation analysis in three osteoblast-related cells (the human fetal osteoblast hFOB 1.19 cell line, Saos 2 osteosarcoma cell line and mesenchymal Stem Cells -MSC-), we identify physical interactions between the proximal part of WNT16 intron 2, shown here to be an active promoter in Saos 2 osteosarcoma cells, and several putative regulatory regions within CPED1. Analysis of previously published RNA-seq data from hFOB cells disclosed low expression of a region located downstream of this promoter. Our results suggest a novel regulatory mechanism of WNT16 in bone, mediated by physical interaction with various enhancer regions within CPED1.
Authors
- Carlos Herrera-Úbeda (ORCID: https://orcid.org/0000-0002-2334-9470)
- Nerea Ugartondo (ORCID: https://orcid.org/0000-0001-9234-9899)
- Neus Roca‐Ayats (ORCID: https://orcid.org/0000-0002-3898-7088)
- Susana Balcells (ORCID: https://orcid.org/0000-0003-1211-1907)
- Núria Martínez‐Gil (ORCID: https://orcid.org/0000-0002-9987-2858)
- Daniel Grinberg (ORCID: https://orcid.org/0000-0001-9859-2590)
- Jordi García‐Fernàndez (ORCID: https://orcid.org/0000-0001-5677-5970)
- Diana Ovejero (ORCID: https://orcid.org/0000-0003-1312-4750)
- Xavier Nogués (ORCID: https://orcid.org/0000-0002-5537-1859)
- Nicola Gritti (ORCID: https://orcid.org/0000-0002-7637-4258)
- Natalia García‐Giralt (ORCID: https://orcid.org/0000-0001-6507-0147)
Institutions
- Instituto de Salud Carlos III (ES)
- European Molecular Biology Laboratory (ES)
- Centre for Biomedical Network Research on Rare Diseases (ES)
- Centro de Investigación Biomédica en Red de Fragilidad y Envejecimiento Saludable (ES)
- Instituto de Investigación de Enfermedades Raras (ES)
- Institut de Recerca Sant Joan de Déu
- Universitat de Barcelona (ES)
Publication Details
- Journal
- Scientific Reports
- Published
- 2025-03-20
- DOI
- https://doi.org/10.1038/s41598-025-93259-z
- Primary Topic
- Connective tissue disorders research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Agència de Gestió d'Ajuts Universitaris i de Recerca