Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies
Neutrophils are essential innate immune cells with unusual anti-microbial properties while dysfunctions of neutrophils lead to severe health problems such as lethal infections. Generation of neutrophils from human induced pluripotent stem cells (hiPSCs) is highly promising to produce off-the-shelf neutrophils for transfusion therapies. However, the anti-microbial potencies of hiPSCs derived neutrophils (iNEUs) remain less documented. Here, we develop a scalable approach to generate iNEUs in a chemical defined condition. iNEUs display typical neutrophil characters in terms of phagocytosis, migration, formation of neutrophil extracellular traps (NETs), etc. Importantly, iNEUs display a strong killing potency against various bacteria such as K.pneumoniae, P.aeruginosa, E.coli and S.aureus. Moreover, transfusions of iNEUs in mice with neutrophil dysfunction largely enhance their survival in lethal infection of different bacteria. Together, our data show that hiPSCs derived neutrophils hold strong anti-microbial potencies to protect severe infections under neutrophil dysfunction conditions.
Authors
- Wanli Ma (ORCID: https://orcid.org/0000-0003-2250-4317)
- Yuchan Mai
- Yanling Zhu
- Xing Hu (ORCID: https://orcid.org/0000-0001-7495-8973)
- Guangjin Pan (ORCID: https://orcid.org/0000-0002-2378-7198)
- Mingquan Wang
- Shuoting Wang
- Baoqiang Kang (ORCID: https://orcid.org/0000-0002-6676-3022)
- Huaisong Lin
- Xiaogang Li (ORCID: https://orcid.org/0000-0001-9255-7559)
- Tianyu Zhang (ORCID: https://orcid.org/0000-0002-3384-3288)
- Junwei Wang (ORCID: https://orcid.org/0000-0001-7526-325X)
- Yan Han (ORCID: https://orcid.org/0000-0002-4952-5153)
- Jian Zhang (ORCID: https://orcid.org/0000-0002-9523-5296)
- Xinrui Guo
- Zhishuai Zhang (ORCID: https://orcid.org/0009-0006-0383-4811)
- Jiaming Gu (ORCID: https://orcid.org/0000-0002-1612-6711)
- Jingxi Huang
- Bo Feng (ORCID: https://orcid.org/0009-0005-0522-952X)
Institutions
- Department of Health (CN)
- Sun Yat-sen University (CN)
- Chinese University of Hong Kong (HK)
- Chinese Academy of Sciences (CN)
- Guangzhou Institutes of Biomedicine and Health (CN)
- Third Affiliated Hospital of Sun Yat-sen University (CN)
- University of Chinese Academy of Sciences (CN)
- University of Hong Kong (HK)
Publication Details
- Journal
- Cell Regeneration
- Published
- 2025-03-20
- DOI
- https://doi.org/10.1186/s13619-025-00227-z
- Citations
- 2
- Primary Topic
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 1.59
Funders
- National Natural Science Foundation of China