Online multimethod platform for comprehensive characterization of monoclonal antibodies in cell culture fluid from injection of crude sample - Incorporation of middle-up and bottom-up workflows
The developed multidimensional platform enables the simultaneous characterization of multiple fractions from a single ProtA-isolated mAb band at intact, middle-up, or bottom-up level using various LC modes at a substantially reduced analysis time.
Authors
- Karin P. M. Geuijen (ORCID: https://orcid.org/0000-0003-2952-170X)
- Rob Haselberg (ORCID: https://orcid.org/0000-0003-1892-1724)
- Marek Vido
- Andrea Gargano (ORCID: https://orcid.org/0000-0003-3361-7341)
- Raya Sadighi (ORCID: https://orcid.org/0009-0001-5008-5036)
- Govert W. Somsen (ORCID: https://orcid.org/0000-0003-4200-2015)
- Sam Wouters (ORCID: https://orcid.org/0000-0003-0919-8466)
- Vera de Kleijne
- Eirini Zioga
Institutions
- Masaryk University (CZ)
- Academic Center for Dentistry Amsterdam (NL)
- Polpharma Biologics (Netherlands) (NL)
- Agilent Technologies (Germany) (DE)
- Hewlett-Packard (Germany) (DE)
- Vrije Universiteit Amsterdam (NL)
Publication Details
- Journal
- Analytica Chimica Acta
- Published
- 2025-03-20
- DOI
- https://doi.org/10.1016/j.aca.2025.343943
- Primary Topic
- Protein purification and stability
- Type
- article
- Field-Weighted Citation Impact
- 0.00