Two new bergamotane-type sesquiterpeniods from the plant endophytic fungus <i>Paraconiothyrium brasiliense</i>

One new bergamotane-type sesquiterpeniod lactam brasilactam A (<b>1</b>) and one new bergamotane-type sesquiterpeniod spiroketal lactone brasiketalolide A (<b>2</b>), along with two known compounds ampullicin (<b>3</b>) and isoampullicin (<b>4</b>) were isolated from the crude extract of the plant endophytic fungus <i>Paraconiothynium brasiliense</i> Verkley. The structures of the new compounds were elucidated on the basis of extensive 2D NMR techniques. The absolute configurations of <b>1</b> and <b>2</b> were assigned by ¹³C NMR and electronic circular dichroism (ECD) calculations. Furthermore, compounds <b>1 </b>-<b> 4</b> were tested for their cytotoxicity against four human cancer cell lines.