Comparative Release of Platelet‐Derived Growth Factor‐AA and Evaluation of Osteoblastic Proliferation of Two Liquid Platelet‐Rich Fibrin Formulations (C‐PRF and I‐PRF): An In Vitro Study

<b>Context:</b> Several biomaterials have been developed in the field of tissue regeneration, in addition to creating a "foreign body reaction," they lack the cellular components that are necessary for the regeneration process and, therefore, do not fulfill their purpose satisfactorily. In this regard, the use of platelet concentrates has gained popularity. However, sufficient scientific evidence is still lacking for the use of platelet concentrates, especially platelet-rich liquid fibrin preparations in combination with xenografts. The results of the present study would give an indication of the advantages of using the combination of xenogenic bone graft in combination with liquid formulations of platelet concentrates in regenerative periodontal therapy. <b>Aims:</b> This in vitro study was performed to compare and evaluate the differential release profile and osteogenic potential of liquid formulations of platelet concentrates, namely, concentrated platelet-rich fibrin (C-PRF) and injectable platelet-rich fibrin (I-PRF). <b>Methods and Materials:</b> The differential release profile of platelet-derived growth factor-AA (PDGF-AA) and osteogenic potential of liquid formulations of platelet concentrates was evaluated using samples collected from four periodontally healthy female volunteers by ELISA and alkaline phosphatase (ALP) assay with the help of human osteosarcoma cell lines (Saos-2). <b>Statistical Analysis:</b> Statistical analysis of growth factor release profile and estimation of ALP activity was performed using the Kruskal-Wallis test to compare the mean difference between the following groups: C-PRF and I-PRF with and without bone graft. Data were analyzed using SPSS Version 21 software. <b>Results and Conclusions:</b> This study clearly shows the advantage of using liquid platelet concentrates in combination with bone grafts compared with bone grafts alone. The study further suggested that the use of C-PRF could be beneficial in regenerative periodontal therapy.