Rapid 3D projection microscopy of neural circuit with two-photon glutamate uncaging

We introduce a rapid dual-view projection imaging technique with two-photon glutamate uncaging capabilities. This method significantly enhances volumetric imaging rates for neural studies over traditional light-sheet imaging. Furthermore, we integrated an independent laser scanning module for two-photon uncaging, allowing for simultaneous synaptic resolution stimulation and 100 Hz volumetric imaging of neural activity in deep tissue. Additionally, we provide imaging results from mouse brain slices undergoing two-photon glutamate uncaging.