Assessing exogenous fatty acid and glucose response in SKOV-3 and HeLa cells using stimulated Raman scattering microscopy with deuterium labeling

The abnormal regulation of metabolism in cancer cells is a well-documented phenomenon, with dysregulation in glucose metabolism being extensively studied. However, the underlying mechanisms that govern this dysregulation remain unclear. It is known that dysregulated glucose and lipid metabolism in cancer is involved in promoting tumorigenesis and metastasis. The focus of this research is on how different cancer cell types regulate their metabolism in response to exogenous glucose and fatty acids. Combining deuterium-labeled Raman probes with stimulated Raman scattering microscopy, we spatially and chemically characterize the metabolic aberrations found in cervical and ovarian cancer cell lines HeLa and SKOV-3, respectively. This study provides new insights into the metabolic adaptations of cancer cells, offering promising avenues for developing targeted anti-cancer therapies.