Advanced hyperspectral SRS imaging for studying epithelial-mesenchymal transition in cancer metastasis

We present a compact and portable stimulated Raman scattering (SRS) imaging system for highspeed, label-free imaging of cells and tissues. Our setup rapidly acquires multispectral datasets with high chemical specificity by scanning the spectral range of 700-3100 1/cm in just 100 ms, providing a tenfold increase in acquisition speed. It allows to visualize cell morphology changes during epithelial-mesenchymal transition (EMT) in cancer cells. SRS offers distinct advantages, providing valuable insights in metabolic processes based on chemical changes without interference of exogenous labels. The novel high-speed SRS system allows rapid screening of morphological and metabolic changes of EMT in cancer cells.