Metabolic NADH FLIM: precise, turnkey quantitative FLIM, FLIM-FRET and more in zen for the Zeiss LSM980

We present NADH metabolic imaging FLIM results and an intuitive turnkey FLIM solution for the Zeiss LSM980 – controlled in the Zen software. Based on the full precision and efficiency of Becker&Hickl GmbH TCSPC technology, data is recorded, sent through the SPCimage NG analysis pipeline, and results are returned to Zen for comparison. Advantages include: direct visualisation of and access to all fit parameters, lifetime analysis and multi-parameter segmentation of 2D/3D data sets, and unsurpassed lifetime resolution with a system IRF down to ~20ps @203 fs bins to resolve even the smallest lifetime differences. Available for new and existing Zeiss LSM980.