High-speed imaging of epithelial-mesenchymal transition in cancer cells by stimulated Raman microscopy
We present a compact and portable stimulated Raman scattering (SRS) imaging system for highspeed, label-free imaging of cells and tissues. Our setup rapidly acquires multispectral datasets with high chemical specificity by scanning the spectral range of 700-3100 1/cm in just 100 ms, providing a tenfold increase in acquisition speed. It allows to visualize cell morphology changes during epithelial-mesenchymal transition (EMT) in cancer cells. SRS offers distinct advantages, providing valuable insights in metabolic processes based on chemical changes without interference of exogenous labels. The novel high-speed SRS system allows rapid screening of morphological and metabolic changes of EMT in cancer cells.
Authors
- Eva Doepker
- Björn Kemper (ORCID: https://orcid.org/0000-0003-3693-9397)
- Jürgen Schnekenburger (ORCID: https://orcid.org/0000-0002-0199-2908)
- Maximilian Brinkmann
- Ramon Droop (ORCID: https://orcid.org/0000-0001-8601-7994)
- Tim Hellwig
- Felix Neumann
- Anke Bonse
- Thomas Wuerthwein
Institutions
- University of Münster (DE)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1117/12.3042709
- Primary Topic
- Spectroscopy Techniques in Biomedical and Chemical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00