A Platform for the Glucose Biosensor Based on Dendritic Gold Nanostructures and Polyaniline-Gold Nanoparticles Nanocomposite

Diabetes mellitus is a pathological condition that requires continuous measurement of glucose concentration in human blood. In this study, two enzymatic mediator-free glucose biosensors based on premodified graphite rod (GR) electrodes were developed and compared. GR electrode modified with electrochemically synthesized dendritic gold nanostructures (DGNS), a cystamine (Cys) self-assembled monolayer (SAM), and glucose oxidase (GOx) (GR/DGNS/Cys/GOx) and GR electrode modified with DGNS, Cys SAM, enzymatically obtained polyaniline (PANI) nanocomposites with embedded 6 nm gold nanoparticles (AuNPs) and GOx (GR/DGNS/Cys/PANI-AuNPs-GOx/GOx) were investigated electrochemically. Biosensors based on GR/DGNS/Cys/GOx and GR/DGNS/Cys/PANI-AuNPs-GOx/GOx electrodes were characterized by a linear range (LR) of up to 1.0 mM of glucose, storage stability of over 71 days, sensitivity of 93.7 and 72.0 μA/(mM cm²), limit of detection (LOD) of 0.027 and 0.034 mM, reproducibility of 13.6 and 9.03%, and repeatability of 8.96 and 8.01%, respectively. The GR/DGNS/Cys/PANI-AuNPs-GOx/GOx electrode was proposed as more favorable for glucose concentration determination in serum due to its better stability and resistance to interfering electrochemically active species. The technological solutions presented in this paper are expected to enable the development of innovative mediator-free enzymatic glucose biosensors, offering advantages for clinical assays, particularly for controlling blood glucose concentration in individuals with diabetes.