Cryopreservation of callus cells of Lupinus angustifolius L. at low temperatures in an electric freezer
With advances in biological sciences, the development of new protocols for cryopreservation of various biological objects becomes increasingly popular. Optimization of modern methods for long-term storage of plant callus tissues, which are difficult to extract and expensive, is of particular relevance. The article assesses the possibility of preserving Lupinus angustifolius L. callus cells for 7 days in a household electric freezer at -80 °C using the traditional cryoprotectant as dimethylsulfoxide. The prospects of the presented method of callus cryopreservation are shown. Research in this area will be continued.
Authors
- A. Hudyakov
- Marta Igorevna Sergushkina (ORCID: https://orcid.org/0000-0002-3113-527X)
- Olga N Solomina (ORCID: https://orcid.org/0000-0002-3307-1951)
- Т. В. Полежаева (ORCID: https://orcid.org/0000-0003-4999-3077)
- O. Zayceva
- O. Shuplecova
Institutions
- Federal Agrarian Scientific Center of the North-East named after NV Rudnitsky (RU)
- Ural Branch of the Russian Academy of Sciences (RU)
- Czech Academy of Sciences, Institute of Physiology (CZ)
Publication Details
- Journal
- Proceedings of the Komi Science Centre of the Ural Division of the Russian Academy of Sciences
- Published
- 2024-11-26
- DOI
- https://doi.org/10.19110/1994-5655-2024-7-127-132
- Primary Topic
- Plant tissue culture and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 0.00