Cryopreservation of callus cells of Lupinus angustifolius L. at low temperatures in an electric freezer

With advances in biological sciences, the development of new protocols for cryopreservation of various biological objects becomes increasingly popular. Optimization of modern methods for long-term storage of plant callus tissues, which are difficult to extract and expensive, is of particular relevance. The article assesses the possibility of preserving Lupinus angustifolius L. callus cells for 7 days in a household electric freezer at -80 °C using the traditional cryoprotectant as dimethylsulfoxide. The prospects of the presented method of callus cryopreservation are shown. Research in this area will be continued.

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Publication Details

Journal
Proceedings of the Komi Science Centre of the Ural Division of the Russian Academy of Sciences
Published
2024-11-26
DOI
https://doi.org/10.19110/1994-5655-2024-7-127-132
Primary Topic
Plant tissue culture and regeneration
Type
article
Field-Weighted Citation Impact
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article

Cryopreservation of callus cells of Lupinus angustifolius L. at low temperatures in an electric freezer

A. Hudyakov, Marta Igorevna Sergushkina, Olga N Solomina, Т. В. Полежаева et al.
Proceedings of the Komi Science Centre of the Ural Division of the Russian Academy of Sciences
Plant tissue culture and regeneration
article

Cryopreservation of callus cells of Lupinus angustifolius L. at low temperatures in an electric freezer

A. Hudyakov, Marta Igorevna Sergushkina, Olga N Solomina, Т. В. Полежаева, O. Zayceva, O. Shuplecova
article en

Abstract

With advances in biological sciences, the development of new protocols for cryopreservation of various biological objects becomes increasingly popular. Optimization of modern methods for long-term storage of plant callus tissues, which are difficult to extract and expensive, is of particular relevance. The article assesses the possibility of preserving Lupinus angustifolius L. callus cells for 7 days in a household electric freezer at -80 °C using the traditional cryoprotectant as dimethylsulfoxide. The prospects of the presented method of callus cryopreservation are shown. Research in this area will be continued.

Proceedings of the Komi Science Centre of the Ural Division of the Russian Academy of SciencesVol. 0(7)
Federal Agrarian Scientific Center of the North-East named after NV Rudnitsky (RU), Ural Branch of the Russian Academy of Sciences (RU), Czech Academy of Sciences, Institute of Physiology (CZ)
Openalex Percentile: Top 77%
Plant tissue culture and regeneration
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