Intrinsically Pro‐Apoptotic Gold Nanoclusters for Optical Tracing and Inhibition of Solid Tumors
Intrinsically theranostic metal nanoclusters are rare unless the stabilizing ligands exhibit therapeutic properties. A promising class of quasi-molecular, near-infrared (NIR) emitting, cytotoxic gold nanoclusters, coined as AXE (Au eXcitable and Eliminable) stabilized through terminal thioester groups on fluorinated, and crosslinked polymers, is presented for simultaneous bioimaging & therapy. Nano Variable Temperature-Electrospray ionization mass spectrometry analysis of these aqueous stable nanoclusters revealed 5 to 7 core gold atoms, with SAXS measurement confirming average size to be under 1 nm, consistent with the theoretical maximum for few atom planar gold clusters. Despite its small size, AXE exhibits a remarkable Stoke shift of ≈470 nm and emission range spanning 700 to 1100 nm. Fluorination notably enhanced the quantum yield by up to twofold, attributed to charge transfer from the fluorinated monomer to the gold core, as indicated by Löwdin charge distribution analysis. The AXE nanocluster demonstrated dose-dependent pro-apoptotic effects on cancer cells while sparing normal cells at lower concentrations. Preclinical evaluation in a breast tumor model confirmed its anticancer efficacy, with intravenous and intraperitoneal administrations significantly inhibiting tumor growth and controlling lung metastasis, surpassing the clinical standard, doxorubicin.
Authors
- Andrew K. Whittaker (ORCID: https://orcid.org/0000-0002-1948-8355)
- Changkui Fu (ORCID: https://orcid.org/0000-0002-2444-607X)
- Christophe Moulin (ORCID: https://orcid.org/0000-0002-8191-1011)
- Nitin Kumar Singhal (ORCID: https://orcid.org/0000-0003-2384-4487)
- Prakash P. Neelakandan (ORCID: https://orcid.org/0000-0003-4150-7994)
- Clothilde Comby‐Zerbino (ORCID: https://orcid.org/0009-0009-0520-9229)
- Md. Ehesan Ali (ORCID: https://orcid.org/0000-0001-6607-5484)
- Sonalika Vaidya (ORCID: https://orcid.org/0000-0001-6857-1705)
- Poonam Sagar (ORCID: https://orcid.org/0000-0002-6694-5272)
- Isabelle Russier‐Antoine
- Rodolphe Antoine (ORCID: https://orcid.org/0000-0001-5682-8550)
- Hemant Hemant
- Asifkhan Shanavas (ORCID: https://orcid.org/0000-0001-7221-7477)
- Pierre‐François Brevet (ORCID: https://orcid.org/0000-0002-9097-0187)
- Rohit Srivastava
- Priyanka Sharma (ORCID: https://orcid.org/0009-0007-1792-644X)
- Hao Yuan (ORCID: https://orcid.org/0009-0005-7798-8066)
- Ruchi Verma (ORCID: https://orcid.org/0000-0002-2895-2279)
- Nisha Mehla (ORCID: https://orcid.org/0009-0006-3232-0588)
Institutions
- Université Claude Bernard Lyon 1 (FR)
- Centre National de la Recherche Scientifique (FR)
- Australian Research Council (AU)
- Indian Institute of Technology Bombay (IN)
- The University of Queensland (AU)
- Institut Lumière Matière (FR)
- BRIC-National Agri-Food and Biomanufacturing Institute (IN)
- Academy of Scientific and Innovative Research (IN)
Publication Details
- Journal
- Advanced Healthcare Materials
- Published
- 2025-03-20
- DOI
- https://doi.org/10.1002/adhm.202405005
- Citations
- 4
- Primary Topic
- Nanocluster Synthesis and Applications
- Type
- article
- Field-Weighted Citation Impact
- 2.07