Kinetic analysis of phenotypic and growth variations in <i>Clostridium autoethanogenum</i> across two xylose concentration ranges

Abstract This study investigated the heterotrophic growth kinetics of Clostridium autoethanogenum in batch cultures using xylose, a hemicellulose hydrolysis product, as the fermentation substrate. The growth data were modeled using the Monod equation. No substrate inhibition was observed within the tested range of xylose concentrations. However, microbial growth deviated from standard Monod kinetics, with distinct growth behavior at different xylose concentration ranges. These findings suggest that C. autoethanogenum utilizes two distinct substrate uptake systems: one with high affinity for xylose and another with lower affinity.