Study of the association between HIF1A and VEGFA gene polymorphisms and ovarian cancer risk in women from Bashkortostan

Background . The major candidate genes for ovarian cancer (BRCA1/2) explain no more than 15–20 % of cases; therefore it is important to focus on the search for new molecular genetic markers. The aim of the study was to analyze the association of rs11549465/HIF1A, rs3025039/VEGFA, and rs2146323/VEGFA polymorphic variants with the risk of developing ovarian cancer in women from the Republic of Bashkortostan. Material and methods . Our research included DNA samples of women with ovarian cancer (n=205) and women without cancer at the time of blood sampling (n=259) from the Republic of Bashkortostan. Genotyping was carried out using the Real Time PCR method based on TaqMan technology. Results . Polymorphic variants, such as rs11549465/HIF1A, rs3025039/VEGFA, and rs2146323/VEGFA were not associated with the risk of developing ovarian cancer in women of the Republic of Bashkortostan. However, the rs11549465/HIF1A polymorphic locus was significantly correlated with the grade of tumor cell differentiation, and the rs3025039/VEGFA was associated with lymph node metastasis. Conclusion . These polymorphic variants may be associated with ovarian cancer prognosis. to confirm this association, it is necessary to conduct research on a large sample size.