Determining the best composition of bacterial cellulose medium made from glycerol and soybean-soaked wastewater for nata de coco production using Taguchi method

The demand for cellulose is increasing every year. Therefore, developing bacterial cellulose (BC) production in the form of nata de coco in Indonesia needs to be done. One of the ways that can be done to support it is by modifying the carbon and nitrogen sources used. In this study, we use soybean-soaked wastewater as the nitrogen source alternative and glycerol as the carbon source alternative. Taguchi methods are used to obtain the best composition of glycerol, soybean-soaked wastewater, and ZA to make the best bacterial cellulose based on its thickness. To verify the best composition obtained by using the Taguchi Method, a confirmatory experiment was carried out. The best composition is soybean-soaked wastewater concentration 20%, glycerol concentration 1%, and ZA concentration 0,2%.