Protective impact of Hesperidin in hyperthyroid-induced Cardiomyopathy in rats

Background: Higher risk of cardiovascular problems is linked to hyperthyroidism. The citrus bioflavonoid hesperidin (HSP) has cardioprotective, anti-inflammatory, and antioxidant impacts.Aim of the study: to illustrate the possible underlying processes and cardioprotective impact of HSP in cardiomyopathy caused by hyperthyroidism.Material and methods: Thirty male albino rats were split into three groups: Hyperthyroid, Hyperthyroid+HSP, and control (10/group). After four weeks rats were subjected to ECG evaluation, LVW/ tibial length, Serum TSH, Serum total T3, T4, Serum LDH, Serum CK-MB, Cardiac MDA, Cardiac SOD, Cardiac TNF-α, Cardiac IL-6, Cardiac genes expression of ANF, Caspase-3 and NF-kB evaluation. Furthermore, cardiac histological and immunohistochemical analyses were performed.Results: There was dramatically elevated heart rate, LVW/ tibial length, serum tT3, serum tT4, serum LDH, serum CK-MB, cardiac MDA, cardiac TNF-α, cardiac IL-6, cardiac ANF gene expression, cardiac Caspase-3 gene expression and cardiac NF-kB gene expression alongside a marked reduction in duration of PR interval and QT interval, serum TSH and cardiac SOD of hyperthyroid group compared to control. HSP dramatically ameliorated hyperthyroid-induced cardiac alterations generated by hyperthyroidism. Conclusion: In hyperthyroid-induced cardiomyopathy, HSP has a cardioprotective effect by enhancing ANF levels and regulating the heart's anti-inflammatory, anti-apoptotic, and antioxidant mechanisms.