Genomic characterization of invasive <i>Neisseria meningitidis</i> in Spain (2011/12–2022/23): expansion of clonal complex 213 and the potential threat to 4CMenB vaccine strain coverage

Invasive meningococcal disease (IMD) is associated with significant global morbidity and mortality and is addressed by conjugated polysaccharide and subcapsular vaccines. In Spain, data on 4CMenB vaccine strain coverage and antimicrobial susceptibility are limited. This study aimed to describe the genomic epidemiology, predict 4CMenB vaccine strain coverage, and assess antimicrobial susceptibility of 323 <i>Neisseria meningitidis</i> isolates causing IMD, collected from 57 Clinical Microbiology Laboratories in Spain over 12 years (2011/12-2022/23). Whole genome sequencing was performed to identify serogroup, clonal complex (cc), and antimicrobial resistance determinants. Vaccine strain coverage for serogroup B (MenB) isolates was predicted using the genetic Meningococcal Antigen Typing System approach. The most prevalent serogroups were B (57.9%), W (21.4%), C (10.4%), and Y (8.4%). MenB predominated throughout most seasons, except during the 2019/20 season when serogroup W peaked. Post-COVID-19 pandemic, MenB remained the most frequent (70.2%). Thirteen cc were identified among MenB isolates, with cc213 being the most prevalent (40.1%). Only 28.9% of MenB isolates were predicted to be covered by 4CMenB, with cc213 showing an exceptionally low coverage rate (5.3%) due to antigenic variants poorly targeted by the vaccine. Notably, cc213 was responsible for twice the proportion of MenB cases in 4CMenB-vaccinated versus unvaccinated. All isolates were susceptible to third generation cephalosporins, and 13.5% showed penicillin resistance. This study highlights the alarming prevalence of cc213 among MenB IMD cases in Spain and the limited 4CMenB coverage against this cc. The disproportionate representation of cc213 in vaccinated individuals underscores its potential to compromise vaccine effectiveness.

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Journal
Emerging Microbes & Infections
Published
2025-03-19
DOI
https://doi.org/10.1080/22221751.2025.2482696
Citations
5
Primary Topic
Bacterial Infections and Vaccines
Type
article
Field-Weighted Citation Impact
5.92
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article

Genomic characterization of invasive Neisseria meningitidis in Spain (2011/12–2022/23): expansion of clonal complex 213 and the potential threat to 4CMenB vaccine strain coverage

M. Ángeles Orellana, Alba Bellés Bellés, Ana Rodrı́guez, Belén Viñado et al.
5 citations
Emerging Microbes & Infections
Bacterial Infections and Vaccines
5.92
article

Genomic characterization of invasive Neisseria meningitidis in Spain (2011/12–2022/23): expansion of clonal complex 213 and the potential threat to 4CMenB vaccine strain coverage

M. Ángeles Orellana, Alba Bellés Bellés, Ana Rodrı́guez, Belén Viñado, Juan José González‐López, Jordi Cámara, Mayli Lung, Begoña Palop, María Dolores Quesada, Daniel Navarro, M. A. Galán‐Ladero, Alba Mir-Cros, Josep Roca-Grande, Carolina Sarvisé, Nieves Larrosa, ALBERT J. MORENO, Amaresh Pérez-Argüello, Carmen Muñoz‐Almagro, Aleix Soler‐Garcia, Enrique Ruíz de Gopegui, Andrea Martín-Nalda, Alba Rivera, Emilia Cercenado, Guillem Puigsech-Boixeda
article en
5 citations

Abstract

Invasive meningococcal disease (IMD) is associated with significant global morbidity and mortality and is addressed by conjugated polysaccharide and subcapsular vaccines. In Spain, data on 4CMenB vaccine strain coverage and antimicrobial susceptibility are limited. This study aimed to describe the genomic epidemiology, predict 4CMenB vaccine strain coverage, and assess antimicrobial susceptibility of 323 <i>Neisseria meningitidis</i> isolates causing IMD, collected from 57 Clinical Microbiology Laboratories in Spain over 12 years (2011/12-2022/23). Whole genome sequencing was performed to identify serogroup, clonal complex (cc), and antimicrobial resistance determinants. Vaccine strain coverage for serogroup B (MenB) isolates was predicted using the genetic Meningococcal Antigen Typing System approach. The most prevalent serogroups were B (57.9%), W (21.4%), C (10.4%), and Y (8.4%). MenB predominated throughout most seasons, except during the 2019/20 season when serogroup W peaked. Post-COVID-19 pandemic, MenB remained the most frequent (70.2%). Thirteen cc were identified among MenB isolates, with cc213 being the most prevalent (40.1%). Only 28.9% of MenB isolates were predicted to be covered by 4CMenB, with cc213 showing an exceptionally low coverage rate (5.3%) due to antigenic variants poorly targeted by the vaccine. Notably, cc213 was responsible for twice the proportion of MenB cases in 4CMenB-vaccinated versus unvaccinated. All isolates were susceptible to third generation cephalosporins, and 13.5% showed penicillin resistance. This study highlights the alarming prevalence of cc213 among MenB IMD cases in Spain and the limited 4CMenB coverage against this cc. The disproportionate representation of cc213 in vaccinated individuals underscores its potential to compromise vaccine effectiveness.

Emerging Microbes & InfectionsVol. 14(1)
Universitat Autònoma de Barcelona (ES), Hospital Sant Joan de Déu Barcelona (ES), Instituto de Salud Carlos III (ES), Hospital de Sant Pau (ES), Bellvitge University Hospital (ES), Hospital Universitari Arnau de Vilanova (ES), Hospital General Universitario Gregorio Marañón (ES), Universitat Internacional de Catalunya (ES), Institut d'Investigació Biomédica de Bellvitge (ES), Complejo Hospitalario Universitario de Santiago (ES), Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (ES), Hospital Universitario 12 De Octubre (ES), Institut de Recerca Biomèdica Catalunya Sud (ES), Vall d'Hebron Institut de Recerca (ES), Hospital Regional Universitario de Málaga (ES), Instituto de Investigación Marqués de Valdecilla (ES), Centro de Investigación Biomédica en Red (ES), Hospital Universitario Reina Sofía (ES), Health Research Institute of the Balearic Islands (ES), Vall d'Hebron Hospital Universitari (ES), Manufacturas Serviplast (Spain) (ES), Biomedical Research Institute (US), Hospital Universitari Joan XXIII de Tarragona (ES), Hospital Universitario Son Espases (ES), Hospital Universitari Germans Trias i Pujol (ES), Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (ES)
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Openalex Percentile: Top 4%
Bacterial Infections and Vaccines
5.92
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