Decoding the genome and epigenome of avian Escherichia coli strains by R10.4.1 nanopore sequencing

Avian pathogenic <i>Escherichia coli</i> (APEC) causes colibacillosis in poultry, which is a very important disease worldwide. Despite well-documented genomic traits and diversity of APEC, its epigenomic characteristics are less understood. This study utilized the high throughput and long-read capabilities of Oxford Nanopore Technology (ONT) to elucidate the genome structures and methylation modifications of three <i>E. coli</i> isolates of avian origin: one intestinal isolate from a healthy wild bird and two systemic isolates from clinically affected chickens. Three complete genomes, each comprising a single chromosome and multiple plasmids were assembled. Diverse virulence-associated genes, antimicrobial resistance genes, mobile genetic elements plasmids and integrons were characterized from the genomes. Despite a limited sample size, our whole genome sequencing (WGS) data highlighted significant genomic diversity among the <i>E. coli</i> strains and enriched repertoire of gene clusters related to APEC pathogenicity. From the epigenetic analysis, multiple methylation modifications, including three N5-methylcytosine (5mC), eight N6-methyladenine (6mA) and two N4-methylcytosine (4mC) modification motifs were identified within all three isolates. Furthermore, common GATC and CCWGG methylation motifs were predominantly distributed within regulatory regions, suggesting a role in epigenetic transcription regulation. This study opens the avenue for future research into pathogenesis, diagnostic and therapeutic strategies of APEC considering epigenetic analysis.