Dynamic cytoskeletal regulation of cell shape supports resilience of lymphatic endothelium
Lymphatic capillaries continuously take up interstitial fluid and adapt to resulting changes in vessel calibre<sup>1-3</sup>. The mechanisms by which the permeable monolayer of loosely connected lymphatic endothelial cells (LECs)<sup>4</sup> maintains mechanical stability remain elusive. Here we identify dynamic cytoskeletal regulation of LEC shape, induced by isotropic stretch, as crucial for the integrity and function of dermal lymphatic capillaries. We found that the oak leaf-shaped LECs showed a spectrum of VE-cadherin-based junctional configurations at the lobular intercellular interface and a unique cytoskeletal organization, with microtubules at concave regions and F-actin at convex lobes. Multispectral and longitudinal intravital imaging of capillary LEC shape and actin revealed dynamic remodelling of cellular overlaps in vivo during homeostasis and in response to interstitial fluid volume increase. Akin to puzzle cells of the plant epidermis<sup>5,6</sup>, LEC shape was controlled by Rho GTPase CDC42-regulated cytoskeletal dynamics, enhancing monolayer stability. Moreover, cyclic isotropic stretch increased cellular overlaps and junction curvature in primary LECs. Our findings indicate that capillary LEC shape results from continuous remodelling of cellular overlaps that maintain vessel integrity while preserving permeable cell-cell contacts compatible with vessel expansion and fluid uptake. We propose a bellows-like fluid propulsion mechanism, in which fluid-induced lumen expansion and shrinkage of LEC overlaps are countered by actin-based lamellipodia-like overlap extension to aid vessel constriction.
Authors
- Mateusz Majda (ORCID: https://orcid.org/0000-0003-3405-2901)
- Sebastian Schürmann (ORCID: https://orcid.org/0000-0001-6497-9754)
- Dietmar Vestweber (ORCID: https://orcid.org/0000-0002-3517-732X)
- Hans Schoofs (ORCID: https://orcid.org/0000-0003-3429-912X)
- Amanda Marks (ORCID: https://orcid.org/0000-0002-3961-8831)
- Marie Jeansson (ORCID: https://orcid.org/0000-0003-1075-8563)
- Sara Barcos
- Sebastián Palacios Martínez (ORCID: https://orcid.org/0000-0001-6626-5798)
- Richard S. Smith (ORCID: https://orcid.org/0000-0001-9220-0787)
- Pipsa Saharinen (ORCID: https://orcid.org/0000-0003-2652-0584)
- Oliver Friedrich (ORCID: https://orcid.org/0000-0003-2238-2049)
- Cord Brakebusch (ORCID: https://orcid.org/0000-0002-9342-1634)
- Rui Benedito (ORCID: https://orcid.org/0000-0003-2458-3055)
- Taija Mäkinen (ORCID: https://orcid.org/0000-0002-9338-1257)
- Britta Engelhardt (ORCID: https://orcid.org/0000-0003-3059-9846)
- Max L.B. Grönloh (ORCID: https://orcid.org/0000-0003-0109-8225)
- Nina Daubel (ORCID: https://orcid.org/0009-0002-3240-4934)
- Konstantin Gaengel (ORCID: https://orcid.org/0000-0002-2682-2833)
- Jaap D. van Buul (ORCID: https://orcid.org/0000-0003-0054-7949)
- Fabian Linsenmeier
- A. Halme (ORCID: https://orcid.org/0009-0001-2835-0796)
- Sarah Schnabellehner (ORCID: https://orcid.org/0000-0003-0558-9339)
Institutions
- John Innes Centre (GB)
- University of Bern (CH)
- Uppsala University (SE)
- University of Copenhagen (DK)
- University of Helsinki (FI)
- Friedrich-Alexander-Universität Erlangen-Nürnberg (DE)
- Norwich Research Park (GB)
- Spanish National Centre for Cardiovascular Research (ES)
- Sanquin (NL)
- Wihuri Research Institute (FI)
- Amsterdam University Medical Centers (NL)
- Max Planck Institute for Molecular Biomedicine (DE)
- University of Amsterdam (NL)
- University of Lausanne (CH)
Publication Details
- Journal
- Nature
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1038/s41586-025-08724-6
- Citations
- 26
- Primary Topic
- Lymphatic System and Diseases
- Type
- article
- Field-Weighted Citation Impact
- 27.18