Characterisation of fibre-rich ingredients obtained from defatted cold-pressed rapeseed cake after protein extraction
Fibre-rich ingredients obtained after protein isolate production from cold-pressed rapeseed meal using alkali extraction at pH 12 with ethanol pretreatment (ingredient 1), and natural deep eutectic solvent (NDES) extraction with choline chloride:urea (ChCl:urea) (ingredient 2) and betaine:citric acid (ingredient 3) were compared in terms of chemical profile, minerals, amino acids, antinutrients, and colour. All ingredients were rich in dietary fibres (46.9 ± 2.36–63.0 ± 1.44 %) and proteins (22.5 ± 0.24–35.5 ± 0.66 %). Minerals, particularly potassium, magnesium, zinc, manganese, and iron, were significantly present. The ChCl:urea system provided the highest lysine and methionine content, with methionine detected only in NDES-extracted ingredients. Glucosinolates were absent in all ingredients. Alkali extraction minimized total phenolic content and tannins, while betaine:citric acid extraction reduced phytates most effectively. NDES systems resulted in better colour parameters. Fibre-rich ingredients exhibit significant potential as functional components for enriching food products, combining high nutritional value with minimized antinutritional factors. • Fibre-rich ingredients were obtained from rapeseed meal using alkali and NDES extractions. • Ingredients were rich in fibers (46.9–63.0 %) and proteins (22.5–35.5 %). • Alkali extraction was the most effective method for minimizing phenolics and tannins. • Betaine:citric acid NDES system reduced phytates most effectively. • NDES systems resulted in better colour parameters.
Authors
- Pavle Jovanov (ORCID: https://orcid.org/0000-0002-6225-2655)
- Danka Dragojlović (ORCID: https://orcid.org/0000-0001-6972-1867)
- Marijana Sakač (ORCID: https://orcid.org/0000-0003-2093-2238)
- Bojana Šarić (ORCID: https://orcid.org/0000-0002-4518-6473)
- Aleksandar Marić (ORCID: https://orcid.org/0000-0001-8889-6267)
- Dalibor Tomić (ORCID: https://orcid.org/0000-0002-2814-1935)
- Branislava Đermanović (ORCID: https://orcid.org/0000-0003-1648-8496)
Institutions
- University of Novi Sad (RS)
Publication Details
- Journal
- LWT
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1016/j.lwt.2025.117671
- Citations
- 2
- Primary Topic
- Phytase and its Applications
- Type
- article
- Field-Weighted Citation Impact
- 3.50