ANALYSIS OF Botryocladia occidentalis (RHODOPHYTA) SULFATED GALACTANS AS MODULATORS OF THROMBIN GENERATION

The cell-wall of Botryocladia occidentalis (Rhodophyta) contains sulfated galactans (BoSGs) with anti- and procoagulant properties, but their effects on thrombin generation (TG) were fewer explored. This study evaluated BoSGs by different extraction times (3, 6, 16 or 24 h) on yield, physical-chemical features and in vitro effects on cephalin-induced TG in 60-fold diluted human plasma using continuous system. Yield of papain extraction from ground matter increased (p<0.05) with time from 5.81±0.71% (BoSGs-3) to 10.03±0.74% (BoSGs-24), with a difference reaching 4.49%. Agarose/polyacrylamide gels electrophoreses revealed profiles of discrete charge density patterns and molecular masses >100kDa among all the wood color-visualized BoSGs after independent staining with toluidin blue or Stains-All vs. glycosaminoglycans standards. Infrared spectroscopy characterized a system of agaran/carrageenan-unrelated BoSGs structure. Regarting TG assay, BoSGs-24 abolished TG at concentration >4.1 μg competing with thrombin, but induced TG in cepahlin-free system. Heparin also inhibited TG, but not stimulated it. Therefore, the technique optimized yield and selected BoSGs with in vitro TG modulation.