Real-time precision opto-control of biochemical processes in living systems

We introduce a novel technology for controlling biochemical processes in living organisms with chemical-specific and site-specific precision. This technology, known as Real-Time Precision Opto-Control (RPOC), employs lasers for simultaneous chemical detection, optical manipulation, and readout of sample responses. RPOC features a fast opto-electronic feedback control system that activates lasers in real-time during scanning, offering several key advantages over traditional methods, including automated target selection, high spatial accuracy, and an uninterrupted laser scanning process throughout treatment. RPOC can be easily integrated into existing laser scanning or stage scanning microscopes with minimal modifications. This transforms a passive imaging system into an active opto-control platform, enabling users to engage with biochemical processes through a diverse range of light-matter interactions. In this talk, I will cover the fundamentals of RPOC, recent technological advancements, and its various biological applications.