Centroid reassignment microscopy (CRM) enables video-rate long-term super-resolution imaging of live samples

Super-resolution (SR) fluorescence imaging is generally incompatible with live-sample imaging. Single Molecule Localization Microscopy (SMLM) provides high resolution but requires multiple raw images and specialized fluorophores. Image Scanning Microscopy (ISM), though efficient, offers limited spatial resolution. We introduce a new SR technique that shares the benefits of ISM and SMLM, and can achieve high-speed, long-term SR imaging with low photon requirements. We apply this to the imaging of neural activity in jellyfish and the monitoring of mitochondrial changes in cancer cells.