New analysis options push the limits of FLIM imaging modalities

The NovaFLIM software boosts FLIM, FLIM-FRET, and anisotropy analysis efficiency for z-stacks, time-lapse series, and tiled images acquired with PicoQuant’s Luminosa confocal microscope. It also supports data from MicroTime 200 and LSM upgrade kits for Nikon, Olympus, and Zeiss systems. With GPU-accelerated batch analysis, processing time is significantly reduced, and advanced export options are available. 1D and 2D histograms of fitted parameters allow for precise ROI definition and comparison of images with different morphologies. Flexible ROI handling based on histograms and phasor plots opens new possibilities. Additionally, NovaISM enables ISM-FLIM analysis using the PDA-23 add-on for Luminosa. Image Scanning Microscopy (ISM) with SPAD arrays enhances resolution by 1.5-1.7x and improves contrast by rejecting out-of-focus light. This leads to a better signal-to-noise ratio, higher lifetime contrast, and faster, gentler imaging of live samples.