Non-invasive characterization of hBM-MSCs using autofluorescence multispectral imaging: future growth and pluripotency
This study developed a non-invasive assay using autofluorescence multispectral imaging (AFMI) for detailed characterization of human bone marrow-derived mesenchymal stromal cells (hBM-MSCs). Cells were cultured, stained for various markers, and imaged to analyze features like intensity, texture, and morphology. Canonical discriminant analysis distinguished different cell groups. AFMI predicted STRO-1 positivity with an AUC of 0.67, and differentiation, cell cycle entry, and senescence with AUCs of 0.66, 0.77, and 0.77, respectively. Population doublings were predicted with Mean Absolute Error of ±1.86 doublings remaining in lifespan. This label-free AFMI method offers valuable insights into cell status and behavior, benefiting clinical applications and research.
Authors
- Robert B. Gilchrist (ORCID: https://orcid.org/0000-0003-1611-7142)
- Ewa M. Goldys (ORCID: https://orcid.org/0000-0003-2470-7118)
- Ayad G. Anwer (ORCID: https://orcid.org/0000-0002-2029-1023)
- Jared M. Campbell (ORCID: https://orcid.org/0000-0003-0163-4251)
- Saabah B. Mahbub (ORCID: https://orcid.org/0000-0002-3320-2499)
- Abbas Habibalahi (ORCID: https://orcid.org/0000-0001-7833-1705)
- Shannon Handley
- Shane T. Grey (ORCID: https://orcid.org/0000-0003-2160-1625)
- Akanksha Bhargava (ORCID: https://orcid.org/0000-0001-8297-6055)
- Stan Gronthos (ORCID: https://orcid.org/0000-0002-6225-3084)
- Adnan Agha (ORCID: https://orcid.org/0000-0002-2704-8931)
- Lindsay E. Wu (ORCID: https://orcid.org/0000-0002-1599-7574)
- Aline Knab (ORCID: https://orcid.org/0009-0006-4715-4252)
- Xiaohu Xu
- Max Mackevicius
- Yuan Tian (ORCID: https://orcid.org/0000-0001-8158-1588)
- Zhilin Lei
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1117/12.3041463
- Primary Topic
- Medical Imaging Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00