Noncontact optical spectroscopy for quantitative measurements of the key metabolic and vascular parameters on small animals in vivo

A non-contact spectroscopy system has been developed to measure diffuse reflectance and fluorescence on small solid tumors in vivo, eliminating distortions caused by inconsistent probe-tissue contact and avoiding infection risks. This system allows for the rapid and precise quantification of key metabolic and vascular parameters, providing a comprehensive view of tumor metabolism and vasculature. The system's capabilities are demonstrated through tissue-mimicking phantom and animal studies. This method offers a real-time, cost-effective approach to cancer research, particularly for studying radiation therapy resistance and recurrence in various cancer models.