Computationally enhanced quantitative phase imaging for label-free organoids and in vivo mouse brains

We propose a label-free deep-imaging method, known as scattering-enabled epi quantitative phase imaging (SEEQPI), for studying 3D organoids and in vivo mouse brains. SEEQPI is a custom-built laser-scanning, common-path, phase-shifting interferometor using long-wavelength illumination. SEEQPI has superior stability and sensitivity in phase measurement and reduces photodamage or heating to living systems. With the proposed method, we will demonstrate the working principle on standard samples, and the phase imaging of cancer spheroids and in vivo mouse brains, compared to colocolized multiphoton imaging.