Two-color laser system for 2-photon FLIM

In two-photon microscopy, simultaneous multi-color imaging provides rich insight into biological samples . Especially, metabolic imaging greatly benefits from simultaneous NADH and FAD measurements. With the new FemtoFiber ultra dual-color from TOPTICA two FemtoFiber ultra laser systems are optically synchronized and allow simultaneous imaging with two different laser colors. In contrast to single TiSa or OPO laser lines, no laser wavelength tuning is required. A shared oscillator design guarantees all-optical synchronization and hence a fixed, defined delay between the two laser colors with minimum jitter. In addition, only a single electronic trigger output serves as reference for TCSPC electronics in Fluorescence lifetime imaging (FLIM) as well as gated detectors.