Polarization-multiplexed holography for label-free microscopy in nonlinear regime
Digital Holography is an imaging method based on interferometry that reconstructs in 3 dimensions both the amplitude and the phase of an electromagnetic field. Since second harmonic generation is a coherent process, it can studied with holography and offers a unique insight in complex, 3D samples with single-shot, label-free and specific microscopy of samples with nonzero second order susceptibilities. With an added multiplexed-polarization study, we collect structural information in biological tissues such as organization of collagen fibrils in porcine cornea.
Authors
- Gilles Tessier (ORCID: https://orcid.org/0000-0003-3558-925X)
- Yannick De Wilde (ORCID: https://orcid.org/0000-0002-5973-1460)
- Valentina Krachmalnicoff (ORCID: https://orcid.org/0000-0002-5484-5338)
- Serena Goldmann
- Marie Fondanèche
Institutions
- Institut de la Vision (FR)
- Université Paris Sciences et Lettres (FR)
- Sorbonne Université (FR)
- ESPCI Paris (FR)
Publication Details
- Published
- 2025-03-19
- DOI
- https://doi.org/10.1117/12.3041692
- Primary Topic
- Digital Holography and Microscopy
- Type
- article
- Field-Weighted Citation Impact
- 0.00