Extremely high-throughput live-cell imaging with the multi-camera-array microscope

This talk details a new microscopic imaging approach for extremely high-throughput live cell video recording. Due to their limited field-of-view, today’s microscopes are extremely inefficient at live-cell recording across large areas (e.g., entire multi-well plates). This is particularly true for 3D recording of in vitro cultures such as organoids. This work presents a multi-camera array microscope (MCAM) that contains dozens of tightly packed imaging systems to simultaneously record video of live-cell dynamics. Associated phase contrast imaging methods that are optimized to operate within a microscope array format produce high-quality label free measurements for automated software analysis.