Intelligent holographic imaging flow cytometry for advanced single-cell analysis

Currently, holographic imaging flow cytometry (HIFC) is one of the most powerful label-free and high throughput imaging modality for single-cell analysis. The combination of HIFC with artificial intelligence can unlock unprecedent challenges, thus opening the way to advanced analysis of biological specimens. Here, several studies will be discusses with the focus of improving target cells identification and classification by exploiting a priori clinical information about the samples. In particular, applications regarding the differential diagnosis of anemia, liquid biopsy, cell phenotyping and drug resistance will be described.