Optical diffraction tomography with multiple wavelengths for highly scattering samples

Optical diffraction tomography (ODT) provides 3D refractive index (RI) information of biological specimens, revealing their structural, mechanical, and biochemical characteristics. To extend the limits of imaging through highly scattering samples, we integrate multi-wavelength illumination into the traditional ODT framework, which typically relies on illumination angle diversity. We explore illumination strategies that combine tilted angles with diverse wavelengths to enhance the accuracy and quality of 3D refractive index reconstructions.