Nonlinear holography: widefield coherent nonlinear imaging and 3D tomography

Non-invasive imaging with high resolution deep within biological materials without the use of harmful ionizing radiation is of great interest in the field of medical imaging. Nonlinear optical microscopy using second harmonic generation (SHG), and third harmonic generation (THG) microscopy provide a powerful contrast mechanism for probing biological systems. Normally, such imaging methods use laser-scanning microscopy to map the scattered SHG or THG light intensity. We introduce the use of quantitative nonlinear optical phase microscopy that is enabled by computational adaptive optics nonlinear holographic microscopy.