Enhanced imaging of thick tissues via digital aberration correction using aberration matrix and tilt-tilt correlation in optical memory effect

Optical aberrations can severely impair the clarity of microscopic images, particularly in cell biology and histopathology. Traditional methods like wavefront shaping and guide star techniques often struggle with biological tissues. We introduce a novel computational adaptive optics method for thick optical samples, utilizing tilt-tilt correlation from the optical memory effect to detect phase discrepancies caused by slight tilts in incident waves. Our technique significantly improves the imaging quality of thick tissues, even under challenging aberration conditions. Additionally, it is highly resistant to sample movement, enhancing imaging accuracy in biomedical applications.