Pixel-reassigned line-scanning microscopy for fast volumetric super-resolution imaging

Super-resolution microscopy has revolutionized the field of biophotonics by revealing detailed 3D biological structures. Nonetheless, the technique is still largely limited by the low throughput and hampered by increased background signals for dense or thick biological specimens. To address this issue, we propose a pixel-reassigned continuous line-scanning microscope for large-scale high-speed 3D super-resolution imaging, which achieves a √2 lateral imaging resolution enhancement and an imaging speed of 3400 pixels/ms on millimeter-scale specimens.